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small rna library construction protocol (dnbseq)  (Beijing Genomics Institute Shenzhen)

 
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    Beijing Genomics Institute Shenzhen small rna library construction protocol (dnbseq)
    Mutation in the catalytic tetrad of Ago2 in AF525 cells has a limited effect on the magnitude of 21 nt vsiRNA reads produced in response to SFV infection . <t>Small</t> <t>RNA</t> analysis of AF525 cells stably expressing eGFP, Ago2, and Ago2mut, and infected with SFV (MOI 5 PFU/cell), analyzed at 24 h.p.i. A , histogram of 21 nt vsiRNAs that mapped to the SFV genome (positive) or antigenome (negative) with colors indicating the nt prevalence of the first base for each read length. Shown as mean % mapped reads (Y axis, percentage reads) from three independent experiments. Error bars show the standard error of the mean. B , coverage of SFV-derived 21 nt vsiRNAs over the viral genome, genomic ( magenta ) or antigenomic ( cyan ) sense RNAs are shown (Y axis, vsiRNA reads per million) from three independent experiments. Error bars show the standard error of the mean. C , mean number of 21 nt viral RNAs per million reads mapping to the SFV genome ( magenta , +) and antigenome ( cyan , −) of all treatments. The graph indicates a mean value of three (n = 3) independent repeats with the range of values given. Ordinary one-way ANOVA with Tukey's multiple comparisons test was used to compare groups. Here, ns: insignificant ∗ p ≤ 0.05, and ∗∗∗ p ≤ 0.0001. GenBank ID for SFV: KP699763.
    Small Rna Library Construction Protocol (Dnbseq), supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+rna+library+construction+protocol+%28dnbseq%29/pmc11968273-204-0-10?v=Beijing+Genomics+Institute+Shenzhen
    Average 90 stars, based on 1 article reviews
    small rna library construction protocol (dnbseq) - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "The catalytic tetrad of Aedes aegypti argonaute 2 is critical for the antiviral activity of the exogenous siRNA pathway"

    Article Title: The catalytic tetrad of Aedes aegypti argonaute 2 is critical for the antiviral activity of the exogenous siRNA pathway

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.108332

    Mutation in the catalytic tetrad of Ago2 in AF525 cells has a limited effect on the magnitude of 21 nt vsiRNA reads produced in response to SFV infection . Small RNA analysis of AF525 cells stably expressing eGFP, Ago2, and Ago2mut, and infected with SFV (MOI 5 PFU/cell), analyzed at 24 h.p.i. A , histogram of 21 nt vsiRNAs that mapped to the SFV genome (positive) or antigenome (negative) with colors indicating the nt prevalence of the first base for each read length. Shown as mean % mapped reads (Y axis, percentage reads) from three independent experiments. Error bars show the standard error of the mean. B , coverage of SFV-derived 21 nt vsiRNAs over the viral genome, genomic ( magenta ) or antigenomic ( cyan ) sense RNAs are shown (Y axis, vsiRNA reads per million) from three independent experiments. Error bars show the standard error of the mean. C , mean number of 21 nt viral RNAs per million reads mapping to the SFV genome ( magenta , +) and antigenome ( cyan , −) of all treatments. The graph indicates a mean value of three (n = 3) independent repeats with the range of values given. Ordinary one-way ANOVA with Tukey's multiple comparisons test was used to compare groups. Here, ns: insignificant ∗ p ≤ 0.05, and ∗∗∗ p ≤ 0.0001. GenBank ID for SFV: KP699763.
    Figure Legend Snippet: Mutation in the catalytic tetrad of Ago2 in AF525 cells has a limited effect on the magnitude of 21 nt vsiRNA reads produced in response to SFV infection . Small RNA analysis of AF525 cells stably expressing eGFP, Ago2, and Ago2mut, and infected with SFV (MOI 5 PFU/cell), analyzed at 24 h.p.i. A , histogram of 21 nt vsiRNAs that mapped to the SFV genome (positive) or antigenome (negative) with colors indicating the nt prevalence of the first base for each read length. Shown as mean % mapped reads (Y axis, percentage reads) from three independent experiments. Error bars show the standard error of the mean. B , coverage of SFV-derived 21 nt vsiRNAs over the viral genome, genomic ( magenta ) or antigenomic ( cyan ) sense RNAs are shown (Y axis, vsiRNA reads per million) from three independent experiments. Error bars show the standard error of the mean. C , mean number of 21 nt viral RNAs per million reads mapping to the SFV genome ( magenta , +) and antigenome ( cyan , −) of all treatments. The graph indicates a mean value of three (n = 3) independent repeats with the range of values given. Ordinary one-way ANOVA with Tukey's multiple comparisons test was used to compare groups. Here, ns: insignificant ∗ p ≤ 0.05, and ∗∗∗ p ≤ 0.0001. GenBank ID for SFV: KP699763.

    Techniques Used: Mutagenesis, Produced, Infection, Stable Transfection, Expressing, Derivative Assay



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    small rna library construction protocol (dnbseq)  (Beijing Genomics Institute Shenzhen)
    90
    Beijing Genomics Institute Shenzhen small rna library construction protocol (dnbseq)
    Mutation in the catalytic tetrad of Ago2 in AF525 cells has a limited effect on the magnitude of 21 nt vsiRNA reads produced in response to SFV infection . <t>Small</t> <t>RNA</t> analysis of AF525 cells stably expressing eGFP, Ago2, and Ago2mut, and infected with SFV (MOI 5 PFU/cell), analyzed at 24 h.p.i. A , histogram of 21 nt vsiRNAs that mapped to the SFV genome (positive) or antigenome (negative) with colors indicating the nt prevalence of the first base for each read length. Shown as mean % mapped reads (Y axis, percentage reads) from three independent experiments. Error bars show the standard error of the mean. B , coverage of SFV-derived 21 nt vsiRNAs over the viral genome, genomic ( magenta ) or antigenomic ( cyan ) sense RNAs are shown (Y axis, vsiRNA reads per million) from three independent experiments. Error bars show the standard error of the mean. C , mean number of 21 nt viral RNAs per million reads mapping to the SFV genome ( magenta , +) and antigenome ( cyan , −) of all treatments. The graph indicates a mean value of three (n = 3) independent repeats with the range of values given. Ordinary one-way ANOVA with Tukey's multiple comparisons test was used to compare groups. Here, ns: insignificant ∗ p ≤ 0.05, and ∗∗∗ p ≤ 0.0001. GenBank ID for SFV: KP699763.
    Small Rna Library Construction Protocol (Dnbseq), supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+rna+library+construction+protocol+%28dnbseq%29/pmc11968273-204-0-10?v=Beijing+Genomics+Institute+Shenzhen
    Average 90 stars, based on 1 article reviews
    small rna library construction protocol (dnbseq) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Mutation in the catalytic tetrad of Ago2 in AF525 cells has a limited effect on the magnitude of 21 nt vsiRNA reads produced in response to SFV infection . Small RNA analysis of AF525 cells stably expressing eGFP, Ago2, and Ago2mut, and infected with SFV (MOI 5 PFU/cell), analyzed at 24 h.p.i. A , histogram of 21 nt vsiRNAs that mapped to the SFV genome (positive) or antigenome (negative) with colors indicating the nt prevalence of the first base for each read length. Shown as mean % mapped reads (Y axis, percentage reads) from three independent experiments. Error bars show the standard error of the mean. B , coverage of SFV-derived 21 nt vsiRNAs over the viral genome, genomic ( magenta ) or antigenomic ( cyan ) sense RNAs are shown (Y axis, vsiRNA reads per million) from three independent experiments. Error bars show the standard error of the mean. C , mean number of 21 nt viral RNAs per million reads mapping to the SFV genome ( magenta , +) and antigenome ( cyan , −) of all treatments. The graph indicates a mean value of three (n = 3) independent repeats with the range of values given. Ordinary one-way ANOVA with Tukey's multiple comparisons test was used to compare groups. Here, ns: insignificant ∗ p ≤ 0.05, and ∗∗∗ p ≤ 0.0001. GenBank ID for SFV: KP699763.

    Journal: The Journal of Biological Chemistry

    Article Title: The catalytic tetrad of Aedes aegypti argonaute 2 is critical for the antiviral activity of the exogenous siRNA pathway

    doi: 10.1016/j.jbc.2025.108332

    Figure Lengend Snippet: Mutation in the catalytic tetrad of Ago2 in AF525 cells has a limited effect on the magnitude of 21 nt vsiRNA reads produced in response to SFV infection . Small RNA analysis of AF525 cells stably expressing eGFP, Ago2, and Ago2mut, and infected with SFV (MOI 5 PFU/cell), analyzed at 24 h.p.i. A , histogram of 21 nt vsiRNAs that mapped to the SFV genome (positive) or antigenome (negative) with colors indicating the nt prevalence of the first base for each read length. Shown as mean % mapped reads (Y axis, percentage reads) from three independent experiments. Error bars show the standard error of the mean. B , coverage of SFV-derived 21 nt vsiRNAs over the viral genome, genomic ( magenta ) or antigenomic ( cyan ) sense RNAs are shown (Y axis, vsiRNA reads per million) from three independent experiments. Error bars show the standard error of the mean. C , mean number of 21 nt viral RNAs per million reads mapping to the SFV genome ( magenta , +) and antigenome ( cyan , −) of all treatments. The graph indicates a mean value of three (n = 3) independent repeats with the range of values given. Ordinary one-way ANOVA with Tukey's multiple comparisons test was used to compare groups. Here, ns: insignificant ∗ p ≤ 0.05, and ∗∗∗ p ≤ 0.0001. GenBank ID for SFV: KP699763.

    Article Snippet: Small RNA library construction and sequencing were undertaken at BGI (Beijing Genomics Institute) using their Small RNA Library Construction Protocol (DNBSEQ).

    Techniques: Mutagenesis, Produced, Infection, Stable Transfection, Expressing, Derivative Assay